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目的探讨经过重复性“饥饿/再投喂”饲喂方式处理的大鼠再摄取高脂食物时,与体重控制和能量平衡调节有关的瘦素(leptin)和一系列神经内分泌因子及激素的表达变化。方法设计“重复性饥饿1天/再投喂1天,持续6周”的饲喂方式(基础饲料)处理实验组(RFR),实验-高脂饲料组大鼠(RFR-LF/HF)另6周改为每天喂以高脂饲料。对照组随机分为3组,对照组喂基础饲料6周后处死,高脂饲料组改用高脂饲料,基础饲料组继续用基础饲料喂养至12周。比较各组大鼠体重、Lee’s指数和脂体比的差异,检测血脂、血清生长激素、甲状腺素、瘦素、胰岛素和血浆促肾上腺皮质激素含量,应用RT-PCR法检测下丘脑神经肽Y(NPY)、前阿黑皮素原(POMC)mRNA的表达水平。结果 RFR-LF/HF组Lee’s指数、脂体比、血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、瘦素和胰岛素水平均显著低于高脂饲料组而高于基础饲料组,下丘脑NPY mRNA表达水平显著高于高脂饲料组和基础饲料组,POMC mRNA表达水平显著低于高脂饲料组和基础饲料组。结论重复性“饥饿/再投喂”饲喂处理可降低高脂饮食诱导的肥胖程度,减轻瘦素抵抗和胰岛素抵抗,但会引起中枢神经内分泌肽表达的极度紊乱。
Objective To investigate the effect of leptin and a series of neuroendocrine factors and hormones on weight control and energy balance regulation in rats fed repeated “starvation / refeeding” feeding on high fat diet Changes in expression. METHODS Design RFR and RFR-LF / HF rats were fed with experimental group (RFR), experimental group (fed with HFR for 1 day / re-fed for 1 day for 6 weeks) Another 6 weeks changed to high-fat feed every day. The control group were randomly divided into three groups, the control group fed with basal diet after 6 weeks of sacrifice, high-fat diet group to high-fat diet, basal diet group continued to feed with basic diet to 12 weeks. The body weight, Lee’s index and lipid ratio were compared between groups to detect the content of serum lipid, serum growth hormone, thyroid hormone, leptin, insulin and plasma adrenocorticotropic hormone. RT-PCR was used to detect the hypothalamic neuropeptide Y ( NPY), pre-pro-melanocortin (POMC) mRNA expression levels. Results The Lee’s index, body fat ratio, serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), leptin and insulin in RFR-LF / HF group were significantly lower than those in high fat diet group The level of NPY mRNA expression in the hypothalamus was significantly higher than that in the high-fat diet group and the basal diet group, and the POMC mRNA expression level was significantly lower than the high-fat diet group and the basic diet group. Conclusions Repeated “starvation / refeeding” feeding treatment can reduce the obesity induced by high-fat diet, reduce leptin resistance and insulin resistance, but cause extreme disturbance of the expression of endocrine peptides in central nervous system.