【目的】探讨3株联合固氮菌单株或组合接种对甘蔗组培苗的促生效应。【方法】采用室内盆栽试验,分别设单菌株(11-31,07-98和08-200)及其4个不同菌株组合共7个接菌处理接种新台糖22号(ROC22)组培苗,设1个不接菌处理为对照,接种86d后比较不同处理对甘蔗生物量、株高、氮磷含量和根系活力等生理生化性状的影响。【结果】7个接菌处理对甘蔗植株的生物量、株高、氮磷含量和根系活力等生理生化性状均有不同程度的提高,其中处理Ⅶ的促生效应最为显著,该处理的植株株高和生物量分别比对照增长25.23%和60.79%;植株总氮含量最高的是处理Ⅳ,比对照增长45.79%;含磷量增长最显著的是处理Ⅶ,比对照增长76.29%。【结论】联合固氮菌单株或组合接种对甘蔗生长、氮磷元素的累积和生理生化性状都有不同程度的促进作用,但不同处理对甘蔗的促生效应有显著差异;多菌株混合接种的促生效应不一定会比单菌株接种高,具体效果因固氮菌种类和组合而异。 【Objective】 The purpose of this study was to investigate the promoting effect of three strains of azotobacter alone or inoculation on tissue culture seedlings of sugarcane. 【Method】 The potted plants of ROC22 (ROC22) were inoculated with a single strain (11-31, 07-98 and 08-200) and four different strains in total of seven inoculation, respectively. The effects of different treatments on the physiological and biochemical characters such as biomass, plant height, N and P content and root activity of sugarcane were compared after one day of inoculation for 86 days. 【Result】 Seven inoculation treatments enhanced the physiological and biochemical characters such as biomass, plant height, N and P content and root activity of sugarcane plants to varying degrees, of which treatment VII had the most significant promoting effect. The plant strains High and biomass increased by 25.23% and 60.79% respectively compared with the control; the highest total nitrogen content of plant was treated Ⅳ, 45.79% more than that of control; the most significant increase of phosphorus content was treated Ⅶ, 76.29% more than that of control. 【Conclusion】 The combination of azotobacter alone or combined inoculation could promote the growth of sugarcane, the accumulation of nitrogen and phosphorus and the physiological and biochemical characters promoted by different degrees, but there were significant differences in promoting growth of sugarcane by different treatments. The pro-survival effect is not necessarily higher than the single strain inoculation, the specific effect due to Azotobacter species and combinations vary.