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目的: 通过调节辣椒素受体1(VR1)基因表达,对VR1 基因的功能及疼痛的基因治疗进行基础研究。方法: 应用RNA干扰(RNAi)技术干扰VR1基因在猴肾Cos-7细胞系中的表达。结果:应用RNAi技术特异性抑制了VR1基因在Cos -7细胞中的表达。随着小片段干扰性RNA(siRNA)浓度的增加,VR1 蛋白的表达量逐渐减少。结论:应用RNAi技术可抑制VR1基因的表达,为开发更有效、安全、合理的镇痛药提供实验依据。
OBJECTIVE: To study the gene therapy of VR1 gene function and pain by regulating the expression of capsaicin receptor 1 (VR1) gene. Methods: RNA interference (RNAi) was used to interfere with the expression of VR1 gene in monkey kidney Cos-7 cell line. Results: The RNAi technology specifically inhibited the expression of VR1 gene in Cos-7 cells. With the increase of small interfering RNA (siRNA) concentration, the expression of VR1 protein gradually decreased. Conclusion: RNAi can inhibit the expression of VR1 gene and provide experimental basis for the development of more effective, safe and reasonable analgesics.