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为方便有效地检测黄酮类物质的含量,使用碳纳米管和石墨烯制备新型修饰电极,用铁氰化钾溶液作探针溶液考察电极的电化学性能,证明修饰后的电极具有较好的电活性。使用修饰电极结合循环伏安法和差分脉冲伏安法2种电化学方法对典型黄酮类化合物槲皮素进行分析检测。检测结果表明:使用该电极在对槲皮素进行检测可以表现出很好的响应性能。在pH为4.5的Brittion-Robinson(B-R)缓冲液中,槲皮素的差分脉冲测试峰电流值与其浓度于1×10~(-7)~1×10~(-5) mol/L存在较好的线性关系,线性回归方程为ipa=-1.447c-0.325,最低检出限为2×10~(-8) mol/L(S/N=3),修饰电极检测简便,灵敏度高,检出极限较低。应用该方法对造粒型苦荞茶中的槲皮素含量进行测定,结果表明:修饰电极在黄酮类化合物实际样品的检测分析中使用,测量结果可靠、准确,具备一定的实际应用价值。
In order to detect the content of flavonoids conveniently and effectively, a new type of modified electrode was prepared by using carbon nanotubes and graphene. The electrochemical performance of the electrode was investigated by using potassium ferricyanide solution as probe solution. active. Quercetin, a typical flavonoid, was analyzed by two electrochemical methods, modified electrode combined with cyclic voltammetry and differential pulse voltammetry. The test results show that the electrode can be used in the detection of quercetin can show good response performance. In the Brittion-Robinson (BR) buffer solution with pH 4.5, the peak current value of quercetin differential pulse with its concentration in the range of 1 × 10 -7 -1 × 10 -5 mol / L The linear regression equation was ipa = -1.447c-0.325, with the lowest detection limit of 2 × 10 -8 mol / L (S / N = 3). The modified electrode was simple and sensitive Out of the lower limit. The method was used to determine the content of quercetin in the granulated buckwheat tea. The results showed that the modified electrode was used in the detection and analysis of the actual samples of flavonoids, and the measurement results were reliable and accurate and had certain practical value.