论文部分内容阅读
健康成年杂种狗20只,随机分为3组:假手术对照组(n=6)、急性胰腺炎组(n=8)和胰腺炎用氯喹肌注顶处理组(n=6).胰腺炎组和氯喹组由主胰管注射自身胆汁1 ml/kg复制急性出血坏死性胰腺炎模型。结果表明,胰腺炎组肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)中肺泡Ⅱ型细胞和多形核白细胞显著增加,BALF及其细胞磷脂总量下降,其中溶血卵磷脂比例增高,卵磷脂和双饱和卵磷脂比例降低.肺匀浆磷脂总量无显著变化,但溶血卵磷脂增加,卵磷脂和双饱和卵磷脂减少.BALF及从中萃取的磷脂的表面活性显著降低.预先肌注氯喹,能显著改善BALF细胞分类和部分磷脂成分的变化,但对BALF及其磷脂的表面活性无显著影响.提示胰腺炎狗肺表面活性物质的损伤与磷脂酶A_2有关,氯喹有防治作用。
Twenty healthy adult mongrel dogs were randomly divided into three groups: sham operation control group (n = 6), acute pancreatitis group (n = 8) and pancreatitis treated with chloroquine intramuscular injection (n = 6) Group and chloroquine group by the main pancreatic duct injection of its own bile 1 ml / kg replication model of acute hemorrhagic necrotizing pancreatitis. The results showed that the number of alveolar type II cells and polymorphonuclear leukocytes in bronchoalveolar lavage fluid (BALF) increased significantly, the total amount of phospholipids in BALF and its cells decreased, the proportion of lysophosphatidylcholine increased, lecithin and bis The proportion of saturated lecithin decreased.The total amount of phospholipids in lung homogenate had no significant change, but lysophosphatidylcholine increased, lecithin and double-saturated lecithin decreased.BALF and the extraction of phospholipids from the surface activity was significantly reduced.Pre-intramuscular chloroquine, can significantly Improve the classification of BALF cells and the changes of some phospholipid components, but have no significant effect on the surface activity of BALF and its phospholipids, suggesting that the injury of canine pulmonary surfactant in pancreatitis is related to phospholipase A_2, and chloroquine has a preventive and therapeutic effect.