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目的应用AmpFISTR IdentifilerTM和STRtyper-10F/G PCR扩增试剂盒,检测195例亲子鉴定案例,分析2个系统在单亲亲子鉴定中的应用价值。方法采用Chelex-100法提取全血基因组DNA,通过IdentifilerTM和STRtyper荧光标记试剂盒对24个STR基因座进行检测,判定基因分型。结果 195例单亲亲子鉴定中,认定亲生血缘关系182例(93.33%),累计PI值大于或等于10 000,RCP>99.999 9%。排除亲生血缘关系13例(6.67%),排除STR位点数5~16范围。在IdentifilerTM检测系统中,1个案例的vWA出现一步突变,1/182(0.55%),其他基因座检测结果均符合遗传规律。结论应用Identifiler和STRtyper系统的24个STR基因座进行DNA亲权鉴定,能准确地提高亲子鉴定判断结果,有效力地避免错误判性发生。
Objective To detect 195 cases of paternity test using AmpFISTR IdentifilerTM and STRtyper-10F / G PCR amplification kit, and to analyze the value of the two systems in single-parent paternity testing. Methods Chelex-100 method was used to extract genomic DNA from whole blood. The genotypes of 24 STR loci were detected by using IdentifilerTM and STRtyper fluorescent labeling kit. Results Among 195 single parents paternity tests, 182 cases (93.33%) were identified as having inborn blood relationship. The cumulative PI value was greater than or equal to 10 000 and RCP> 99.999 9%. Thirteen cases (6.67%) were excluded from natural blood relationship, and the range of STR locus was excluded from 5 to 16. In the IdentifilerTM detection system, one case of vWA showed a one-step mutation, 1/188 (0.55%), and the other loci test results were in accordance with the genetic law. Conclusion Applying the 24 STR loci of Identifiler and STRtyper to DNA paternity testing can accurately improve the results of paternity testing and effectively avoid false judgments.