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目的:探讨TPA对原代白血病细胞的生长抑制和诱导分化作用。方法:取28例白血病患者原代白血病细胞,分别应用TPA、RA和TPA联合RA进行药物干预培养,用MTT比色法测定各组细胞活力,并观察细胞形态学和组织化学变化。结果:干预培养的原代白血病细胞均出现不同程度的生长抑制。TPA组和TPA+RA组的28例白血病细胞均在12 h~48 h后出现聚集现象。23例ANLL细胞中TPA组19例出现细胞贴壁和胞质丝状突起,TPA+RA组22例出现细胞贴壁和丝状突起。TPA组和TPA+RA组的NAE活性增强。5例ALL均出现细胞聚集反应,NAE染色阴性。结论:TPA可以抑制体外原代急性白血病细胞的生长和增生,并有促使其向单核巨噬细胞分化作用,联合应用RA其抑制效果更明显。
Objective: To investigate the effect of TPA on the growth inhibition and differentiation of primary leukemia cells. Methods: Twenty-eight primary leukemic cells from patients with leukemia were treated with TPA, RA, TPA and RA respectively. The viability of the cells was measured by MTT assay and the morphological and histochemical changes were observed. Results: The primary cultured leukemic cells showed different degrees of growth inhibition. Twenty-eight leukemia cells in TPA group and TPA + RA group appeared aggregation after 12 h to 48 h. In 23 cases of ANLL cells TPA group appeared in 19 cases of adherent cells and cytoplasmic filamentous protrusions, TPA + RA group of 22 cases of adherent cells and filaments. NAA activity increased in TPA group and TPA + RA group. 5 cases of ALL cells were aggregated, NAE staining negative. CONCLUSION: TPA can inhibit the growth and proliferation of primary acute leukemia cells in vitro and induce its differentiation into mononuclear macrophages. Combined with RA, its inhibitory effect is more obvious.