论文部分内容阅读
采用两种不同的生长素及处理方法，诱导西洋梨Ｃ４试管苗生根。试管生根法最适的ＩＢＡ浓度为１．２ｍｇ／Ｌ，生根率为６１．９％；ＮＡＡ浓度０．８ｍｇ／Ｌ效果较好，生根率为５３．７％。得到的生根苗生长健壮，发育良好，茎粗达１～２ｍｍ。用一定浓度的ＩＢＡ、ＮＡＡ浸枝处理，前者促根效果较好，ＩＢＡ３．０ｍｇ／Ｌ，生根率达６７．６％；同样浓度的ＮＡＡ促根效果较差，生根率只有３０％，并且这种方法得到的生根苗生长较弱，茎粗１ｍｍ左右。将促进生根的茎段适时转入不含激素的培养基中，不定根的形成部位不产生愈伤组织，从而为试管苗移栽奠定了良好的基础
Two different auxins and treatments were used to induce the rooting of C4 pears in Pear. The optimum concentration of IBA for rooting was 1.2mg / L, and the rooting rate was 61.9%. The NAA concentration of 0.8mg / L was better and the rooting rate was 53.7%. The rooting seedlings obtained grew robustly and developed well with a stem diameter of 1 ~ 2mm. With a certain concentration of IBA, NAA soaking branches, the former to promote rooting better, IBA3.0mg / L, the rooting rate of 67.6%; the same concentration of NAA rooting effect is poor, only 30% of the rooting rate, and this Rootstock seedlings obtained by the method of growth is weak, about 1mm stem diameter. Will promote the rooting of stems timely transfer to hormone-free medium, adventitious root formation site does not produce callus, thus transplanting test tube seedlings laid a good foundation