论文部分内容阅读
研究半边旗（Ｐｔｅｒｉｓ ｓｅｍｉｐｉｎｎａｔａ Ｌ， ＰｓＬ）有效成分５Ｆ，６Ｆ，Ａ对ＨＬ－６０细胞ＤＮＡ拓扑异构酶活性和细胞周期的影响．方法：应用ｐＢＲ３２２质粒ＤＮＡ作为底物测定酶的活性；细胞周期用流式细胞仪测定；应用噻唑蓝（ＭＩＴ）法测定药物对细胞生长的抑制率．结果：５Ｆ，６Ｆ，Ａ均能够抑制ＤＮＡ拓扑异构酶Ⅰ，Ⅱ的活性，化合物６Ｆ作用细胞２４ ｈ后，可升高 Ｓ期和 Ｇ２／Ｍ期细胞，同时降低Ｇ０／Ｇ１期细胞，低浓度６Ｆ（５７．８和 １１５．６ｎｍｏｌ·Ｌ－１）与金雀异黄素（Ｇｅｎ）合用可增强它对ＨＬ－６０细胞的杀伤作用， ｑ＞１．１５．结论： ５Ｆ， ６Ｆ和 Ａ明显抑制ＨＬ－６０细胞ＤＮＡ拓扑异构酶的活性；６Ｆ阻断细胞于 Ｇ２／Ｍ期，增强Ｇｅｎ对 ＨＬ－６０细胞的杀伤作用。
To study the effect of 5F, 6F, A active ingredient Pteris semipinnata L (PsL) on DNA topoisomerase activity and cell cycle in HL-60 cells. Methods: Enzyme activity was determined by using pBR322 plasmid DNA as a substrate. The cell cycle was measured by flow cytometry. The inhibitory rate of the drug on cell growth was determined by MTT assay. Results: 5F, 6F and A inhibited the activity of DNA topoisomerase Ⅰ and Ⅱ. After treated with compound 6F for 24 h, the cells in S phase and G2 / M phase increased and the cells in G0 / G1 phase decreased The combined effect of 6F (57.8 and 115.6nmol·L-1) and genistein (Gen) could enhance the killing effect on HL-60 cells with q> 1.15. CONCLUSION: 5F, 6F and A significantly inhibit the DNA topoisomerase activity of HL-60 cells. 6F blocks G2 / M phase and enhances the killing effect of Gen on HL-60 cells.