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目的:观察人成体牙髓细胞体内诱导牙髓组织修复反应的能力。方法:在矿化诱导液作用下,将一定数量级的人牙髓细胞与β-TCP生物陶瓷颗粒进行复合,植入免疫缺陷鼠磨牙穿髓孔处,7d、14d后分别取材进行组织学观察。对照组采用氢氧化钙(Dycal)和空白对照组。结果:组织学观察表明,盖髓术后7d,各组均出现了牙髓细胞向穿髓孔处迁移、聚集。术后14d,牙髓细胞组炎症反应仅局限于穿髓孔处,有明显的修复性牙本质桥形成;Dycal组炎症反应涉及到少量冠髓,有部分矿化的纤维性屏障形成;空白对照组炎症反应涉及了大部分冠髓,仅有弥散的骨样牙本质形成。结论:在矿化诱导液作用下,牙髓细胞具有向成牙本质细胞样细胞定向分化的能力,将牙髓细胞植入鼠磨牙的穿髓孔处,显示其具有良好的维持牙髓活力和诱导修复性牙本质形成的能力。
Objective: To observe the ability of human adult dental pulp cells to induce dental pulp tissue repair reaction in vivo. Methods: A certain amount of human dental pulp cells were mixed with β-TCP bioceramic particles under the action of mineralization inducing fluid and implanted into the perforation of immunocompromised molars. Seven and 14 days later, the specimens were harvested for histological observation. The control group used calcium hydroxide (Dycal) and blank control group. Results: Histological observation showed that, on the 7th day after the pulp capping operation, the dental pulp cells migrated and aggregated into the pulp hole. On the 14th day after operation, inflammatory reaction in dental pulp cell group was confined to the site of the perforation of the pulp and obvious repaired dentin bridge was formed. Inflammatory reaction of Dycal group involved a small amount of coronal pulp with some mineralized fibrous barriers formed. Blank control The inflammatory response involved most of the coronal pulp, with only diffuse osteoid-like dentin formation. CONCLUSION: The dental pulp cells have the ability to differentiate into odontoblast-like cells under the action of mineralization inducing fluid. The dental pulp cells are implanted into the perforation of the mouse molar, which shows that the dental pulp cells have a good ability to maintain dental pulp vitality. Ability to induce reparative dentin formation.