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目的:分析大连市疟疾病例实验室检测结果,为疟疾诊断方法的选择提供理论依据。方法 :收集2015年大连市疟疾患者的血片和血液样本,应用镜检,RDT和巢氏PCR(Nest-PCR)分别对血片和血样进行检测。结果:17份样品镜检检出恶性疟原虫10例,卵形疟原虫1例,阴性6例;RDT检出恶性疟原虫13例,共同抗原阳性者1例,阴性3例,与镜检结果一致率为82.4%(14/17);巢式PCR检出恶性疟原虫13例,阴性4例,与镜检结果一致率为82.4%(14/17),与RDT结果的一致率为94.1%(16/17)。结论:采用镜检和RDT联合应用的方法,能够提高检测的敏感性,巢式PCR可提高虫种鉴定的准确性。
Objective: To analyze the laboratory results of malaria cases in Dalian and provide theoretical basis for the selection of malaria diagnostic methods. Methods: Blood samples and blood samples from malaria patients in Dalian were collected in 2015, and blood samples and blood samples were detected by microscopy, RDT and Nest-PCR respectively. Results: In 17 samples, 10 cases of Plasmodium falciparum, 1 case of Plasmodium ovale and 6 cases of negative were detected by microscopy. 13 cases of Plasmodium falciparum were detected by RDT, 1 case was positive for common antigen and 3 cases were negative, The concordance rate was 82.4% (14/17). Nested PCR was positive in 13 cases of Plasmodium falciparum and negative in 4 cases. The coincidence rate with the microscopic examination was 82.4% (14/17). The coincidence rate with RDT was 94.1% (16/17). Conclusion: The combination of microscopic examination and RDT can improve the sensitivity of the detection, and nested PCR can improve the accuracy of the identification of the species.