采用选择性双步抽提法,配合普通组化及免疫组化法、整装电镜法及中间纤维蛋白电泳扫描定量分析法,对体外培养的高分化鼻咽癌细胞株CNE-1及低分化鼻咽癌细胞株CNE-2Z的中间纤维进行综合研究。结果表明:两株细胞内均含有网状排列的角蛋白纤维,但细胞分化不同,其中间纤维的排列及角蛋白的组成和含量均明显不同。低分化鼻咽癌细胞中间纤维的排列较高分化细胞紊乱,角蛋白含量低,但亚基种类多。 Using selective two-step extraction method, combined with normal histochemical and immunohistochemical methods, electron microscopy and intermediate fibrin electrophoresis scanning quantitative analysis of well-differentiated nasopharyngeal carcinoma cell line CNE-1 and poorly differentiated Intermediate fibers of nasopharyngeal carcinoma cell line CNE-2Z were studied comprehensively. The results showed that both cells contained reticular keratin fibers, but the cell differentiation was different. The arrangement of intervening fibers and the composition and content of keratin were significantly different. Poorly differentiated nasopharyngeal carcinoma cells in the middle of the fiber arrangement of higher differentiated cells disorder, keratin content is low, but many subunits.