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目的：观察ｐ５３效应分子ｐ２１、Ｇａｄｄ４５是否参与人源细胞纺锤体检查点调控。方法：利用基因转染技术构建稳定表达ｐ２１、Ｇａｄｄ４５反义ＲＮＡ的ＭＣＦ－７细胞系，观察纺锤体抑制剂诺考达唑（ｎｏｃｏｄａｚｏｌｅ）是否诱导多倍体细胞产生，细胞纺锤体检查点调控是否异常。结果：诺考达唑诱导２３ｈ、６０ｈ均未观察到稳定表达ｐ２１、Ｇａｄｄ４５反义ＲＮＡ的ＭＣＦ－７细胞多倍体产生，但表达ｐ２１反义ＲＮＡ的ＭＣＦ－７细胞，诺考达唑引起的细胞Ｍ期聚集明显延迟。结论：ｐ２１参与细胞Ｇ２／Ｍ期转换的调控，但人源细胞具有除ｐ５３、ｐ２１外复杂机制调控细胞纺锤体检查点，防止多倍体产生，保持基因组的稳定。
Objective: To observe whether p53 effector molecules p21 and Gadd45 are involved in the regulation of human cell spindle checkpoint. Methods: The MCF-7 cell line stably expressing p21 and Gadd45 antisense RNA was constructed by gene transfection technique. The spindle inhibitor nocodazole was used to induce polyploid cell production. Whether the cell spindle checkpoint was controlled abnormal. Results: Nocodazole induces polyploidy of MCF-7 cells stably expressing p21 and Gadd45 antisense RNA at 23h and 60h. However, MCF-7 cells expressing p21 antisense RNA and nocodazole induced Cell M phase aggregation was significantly delayed. CONCLUSION: p21 is involved in the regulation of G2 / M phase transition in cells. However, human cells have a complex mechanism of p53 and p21 control of cell spindle checkpoint to prevent polyploidy and maintain the stability of the genome.