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设计了蛋白质含世为45%、35%和25%的三种饵料,以鲜活饵料作对照,研究饵料蛋白质对中华绒螫蟹仔蟹(6±0.5g)消化酶活性的影响。分别于饲养后第10天、20天和40天时取其肝胰腺,测定胰蛋白酶和淀粉酶活性,结果表明:(1)肝胰腺胰蛋白酶活性在投喂鲜活饵料时,整个实验过程中无明显差异(P>0.05);三个实验饵料组蛋白质至第20天时对胰蛋白酶活性才产生显著影响,其中25%蛋白饵料组显著低于其它三个饵料组(P<0.05)。(2)投喂鲜活饵料的仔蟹肝胰腺中淀粉酶比活力在第10d时为49.39 U·mg-1,此后逐渐递减;比较三个蛋白饵料组淀粉酶活性,发现淀粉酶活性自第10天起即受饵料蛋白质的显著影响(P<0.05),至第20天时45%蛋白饵料组比活力为23.85 U·mg-1,较其它两个蛋白饵料组及对照组相比显著降低(P< 0.05),而第40天时对照组和25%蛋白饲料组的淀粉酶比活力显著高于45%和35%二个蛋白饵料组(P< 0.05)。(3)胰蛋白酶mRNA丰度以25%蛋白饵料组为最低,仅0.140,与35%蛋白饵料组较为接近(P> 0.05),而对照饵料组和45%蛋白饵料组的mRNA丰度分别为饲料3组的4.5倍和3.5倍,显著高于25%和35%两个蛋白饵料组(P<0.05)。结果提示仔蟹饵料蛋白质对肝胰腺胰蛋白酶具有显著促进作用,mRNA丰度的变化反映了饵料蛋白质水平导致胰蛋白酶活性的变化是由基?
The effects of dietary protein on the activities of digestive enzymes of 6 ± 0.5g crabs were studied with three different diets containing 45%, 35% and 25% of protein content. The hepatopancreas were harvested at day 10, day 20 and day 40 after feeding respectively. The activities of trypsin and amylase were determined. The results showed that: (1) The activities of hepatopancreas trypsin in feeding fresh live bait (P> 0.05). The trypsin activity of the three experimental dietary groups had a significant effect on trypsin activity by day 20, of which the 25% protein diet group was significantly lower than the other three dietary groups (P <0.05). (2) The specific activity of amylase in liver and pancreas fed with fresh feed was 49.39 U · mg-1 on day 10, and then gradually decreased. The amylase activity of three protein diet groups was compared, (P <0.05). The specific activity of 45% protein diet group on day 20 was 23.85 U · mg-1, which was significantly lower than that of the other two protein diet groups and the control group (P <0.05) P <0.05). On the 40th day, the amylase specific activities of the control group and the 25% protein diet group were significantly higher than those of the 45% and 35% two protein diet groups (P <0.05). (3) The mRNA abundance of trypsin was the lowest in the 25% protein diet group, only 0.140, which was close to the 35% protein diet group (P> 0.05), while the mRNA abundance of the control diet group and the 45% protein diet group were 4.5 and 3.5 times higher than that of feed 3 groups, which was significantly higher than that of 25% and 35% (P <0.05). The results suggested that the protein of bait crab had a significant promotion on hepatopancreas trypsin. The change of mRNA abundance reflected that the change of trypsin activity caused by the level of dietary protein was caused by basal diet.