论文部分内容阅读
小菜蛾颗粒体病毒(Plutella xylostella granulovirus,PlxyGV)基因组含有15个杆状病毒晚期表达因子(Lateexpression factor,lef)基因同源物。PlxyGV的14个lef基因(不包括ie-0)预期编码产物与苜蓿银纹夜蛾核多角体病毒(Autographa californica multicapsid nucleopolyhedrovirus,AcMNPV)LEF蛋白的序列相似度为13%～53%。其中,LEF-8、LEF-9和P47在两种病毒之间的相似度较高,分别为49%、53%和46%。为了研究不同杆状病毒种间lef基因的功能相关性,验证PlxyGVlef基因的功能,本文利用AcMNPV的一个瞬时表达实验系统测试PlxyGVlef基因在Sf9细胞中激活AcMNPV晚期启动子控制的报告基因表达的能力。实验结果显示,在其它AcMNPVlef基因都存在的情况下,PlxyGVlef-2能够部分替代AcMNPVlef-2的活性。序列对比结果显示Plx-yGV LEF-2的C端末比其它GV和鳞翅目NPV的LEF-2分别多出约100aa和70aa。
The genome of Plutella xylostella granulovirus (PlxyGV) contains 15 baculovirus late expression factor (lef) homologues. The 14 lef genes (excluding ie-0) of PlxyGV had a sequence similarity of 13% -53% with the predicted LMP protein of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). Among them, LEF-8, LEF-9 and P47 had high similarity between the two viruses, 49%, 53% and 46% respectively. In order to study the functional relationship of lef genes among different baculoviruses and to verify the function of PlxyGVlef gene, we tested the ability of PlxyGVlef gene to activate the expression of the reporter gene controlled by AcMNPV late promoter in Sf9 cells by using a transient expression system of AcMNPV. The experimental results show that PlxyGVlef-2 can partially replace the activity of AcMNPVlef-2 in the presence of other AcMNPVlef genes. Sequence comparison results show that the C-terminus of Plx-yGV LEF-2 is about 100aa and 70aa more than the LEF-2 of other GV and Lepidopteran NPV, respectively.