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以电镜细胞化学的方法,观察了小鼠胆小管酶的分布。观察的12种酶中,NDPase和G6Pase三种酶分布于胆小管微绒毛;AlPase、Na~+-K~+ATPase、Mg~(++)-ATPase、Ca~(++)-ATPase、CMPase、ACase和5＇-Nase等7种酶分布于胆小管微绒毛,也分布于肝细胞邻接面细胞膜和Disse间隙微绒毛;ACPase、细胞色素氧化酶以及线粒体ATPase不分布胆小管微绒毛,也不分布Disse间隙微绒毛和肝细胞膜。据信,胆小管微绒毛上的酶参与胆汁成份的转运,提供转运所需能量以及还可能与某些代谢过程有关。本实验中磷酸水解酶类使用的铈基法及亚铁氰化钾半还原的锇酸后固定法,效果优于铅法。
Electron microscopy cytochemistry method was used to observe the distribution of mouse tubular enzyme. Of the 12 enzymes observed, NDPase and G6Pase were distributed in the villus microvilli; AlPase, Na ~ + -K ~ + ATPase, Mg ~ (++) - ATPase, Ca ~ (++) Seven kinds of enzymes, CMPase, ACase and 5’-Nase, were distributed in the villus microvilli and were also distributed in the hepatocytes adjacent to the cell membrane and Disse gap microvilli; ACPase, cytochrome oxidase and mitochondrial ATPase were not distributed in the villus microvilli , Nor distribution of Disse gap microvilli and hepatocyte membranes. It is believed that the enzymes on the tibral microvilli participate in the transport of biliary components, provide the energy required for transport, and may also be involved in certain metabolic processes. In this experiment, the phosphohydrolases used in the cerium-based method and potassium ferrocyanide semi-reduced osmium acid post-fixation method, the effect is better than the lead method.