论文部分内容阅读
目的探讨寡核苷酸分型(Spoligotyping)用于安徽省结核分枝杆菌临床分离菌株的分型,以初步了解安徽省结核分枝杆菌的基因型特征。方法连续收集安徽省胸科医院391株结核分枝杆菌临床分离菌株,进行Spoligotyping,采用Bio Numerics软件进行聚类分析。结果北京家族占86.19%(337/391),非北京家族占13.81%(54/391),54株非北京家族基因型中,T家族36株(包括T1 22株、T2 10株、T3 5株、T4 1株),占9.72%;H(Haarlem)家族4株,占1.02%;另有14株(3.58%)为New Found型,北京家族菌株对一线药物总的耐药率为23.14%(78/337),非北京家族菌株为27.78%(15/54),经卡方检验两者间的差异无统计学意义(P>0.05)。结论安徽省结核分枝杆菌存在明显的基因多态性,主要流行型为北京家族,北京家族与耐药无明显相关性。
OBJECTIVE: To investigate the genotyping of Mycobacterium tuberculosis isolates in Anhui province by using the method of oligonucleotide typing (Spoligotyping) for clinical isolates of Mycobacterium tuberculosis in Anhui Province. Methods Thirty-one clinical isolates of Mycobacterium tuberculosis from Anhui Thoracic Hospital were collected continuously and spoligotyping. Bio Numerics software was used for cluster analysis. Results The Beijing family accounted for 86.19% (337/391), while the non-Beijing family accounted for 13.81% (54/391). Among the 54 non-Beijing family genotypes, 36 T families (including T1 22, T2 10 and T3 5 , T4 1 strain) accounted for 9.72%, 4 strains of Haarlem family accounted for 1.02%, and 14 strains (3.58%) were New Found type. The total resistance rate of Beijing family strains to first-line drugs was 23.14% 78/337), non-Beijing family strains was 27.78% (15/54). There was no significant difference between them (P> 0.05) by chi-square test. Conclusion Mycobacterium tuberculosis in Anhui Province has obvious genetic polymorphism. The main epidemic type is Beijing family. There is no significant correlation between Beijing family and drug resistance.