现行肝素的效价测定主要分二类:一类为生物测定法,如兔全血法、羊血浆法等。这类方法操作较繁锁、费时,动物来源较不方便,加之动物个体差异性等影响测定的因素较多。于生物测定法有上述缺陷,生产单位迫切需要能找到一简便的方法,代替生物测定法,以利于生产厂对肝素中间体及成品的快速分析。另一类为化学法,现在研究和报导的方法较多,如纸层析法,N-硫酸盐的浊度比色法,络合量法及琼脂糖电泳法等。上述这些方法考虑到仪器及试剂上的困难,所 The prevalence of heparin is mainly divided into two categories: one for the bioassay, such as rabbit whole blood, sheep plasma method. More complicated operation of such methods, time-consuming, less convenient source of animals, combined with animal individual differences and other factors that affect the determination of more. In the bio-assay has the above-mentioned shortcomings, the production unit urgently needs to find a simple method to replace the bioassay, in order to facilitate the rapid analysis of the production plant of heparin intermediates and finished products. The other is chemical method, there are many methods studied and reported now, such as paper chromatography, N-sulfate turbidimetric method, complexation method and agarose electrophoresis. These methods take into account the equipment and reagents on the difficulties