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目的:研究3′-大豆苷元磺酸钠对大鼠脑缺血/再灌注损伤的保护作用,并初步探讨其保护机制与抗氧化作用的关系。方法:大鼠大脑中动脉栓塞,制作局灶性脑缺血/再灌注损伤模型,缺血后10min,于舌下静脉给不同剂量的3′-大豆苷元磺酸钠。再灌注24h后,腹腔静脉取血并分离血清,断头取脑,一组前脑做TTC染色,测定脑梗死体积,另一组左脑制成10%的脑组织匀浆。比色法测血清及脑组织匀浆SOD、LDH的活性及MDA的含量。结果:与模型组相比,各剂量的3′-大豆苷元磺酸钠治疗组脑梗死体积减小;血清及脑组织SOD的活性显著升高、MDA含量均降低;血清LDH活性明显降低,组织LDH活性降低。结论:3′-大豆苷元磺酸钠对大鼠脑缺血/再灌注损伤具有一定的保护作用,其保护机制与3′-大豆苷元磺酸钠提高抗氧化作用、增强氧自由基的清除有关。
Objective: To investigate the protective effect of 3′-daidzein sulfonate on cerebral ischemia/reperfusion injury in rats, and to explore the relationship between its protective mechanism and antioxidation. METHODS: Middle cerebral artery occlusion was performed in rats to establish a model of focal cerebral ischemia/reperfusion injury. 10 min after ischemia, different doses of 3′-daidzein sulfonate sodium were administered in the sublingual vein. After 24 hours of reperfusion, blood was taken from the celiac veins and serum was separated. The brains were decapitated and a group of forebrains were stained for TTC to determine the infarct volume. Another group of left brains was made with 10% brain homogenate. Colorimetry was used to measure the activity of SOD, LDH and the content of MDA in serum and brain homogenate. Results: Compared with the model group, the cerebral infarct volume of each dose of 3′-daidzein sodium sulfonate treatment group decreased; the activity of SOD in serum and brain tissue increased significantly and the content of MDA decreased; the activity of serum LDH decreased significantly. Tissue LDH activity is reduced. Conclusion: 3′-daidzein sulfonate has certain protective effects on cerebral ischemia/reperfusion injury in rats. Its protective mechanism and 3′-daidzein sodium sulfonate improve antioxidation and enhance oxygen free radicals. Clear about.