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从肿瘤细胞株分离的DNA能转化NIH/3T3成纤维细胞,人膀胱,肺、结肠癌细胞株DAN 的转化活性和Harvey或Kirsten鼠肉瘤病毒ras基因在细胞内对应物的突变有关,但人们还不明确这种突变和体内多步骤肿瘤生成作用的关系以及细胞致癌基因活化对实体瘤生成的进一步诱导怍用。本文用DMBA和TPA处理诱发小鼠皮肤肿瘤,原发的是乳头瘤,其中某些自发退化而另一些则发展成可皮下移植的鳞癌.来自三种移植性癌的DNA都能诱发NIH/3T3细胞形成转化灶,其频率仪略低于人EJ膀胱癌细胞株的DNA,后者有Harvey和Kisotcn鼠肉瘤病毒ras基因,分析限制内切酶谱表明小鼠皮肤癌DNA诱发的转化灶存在另一种ras~H基因拷贝,这不是NIH/3T3
The DNA isolated from tumor cell lines can be transformed into NIH/3T3 fibroblasts. The transformation activity of the human bladder, lung and colon cancer cell lines DAN is related to the mutation of the Harvey or Kirsten mouse sarcoma virus ras gene in the intracellular counterparts, but people also The relationship between this mutation and multistep tumorigenesis in vivo and the further induction of solid tumor formation by cellular oncogene activation are unclear. In this article, DMBA and TPA treatment induce skin tumors in mice. Primary papillomas, some of which spontaneously degenerate, and others develop into subcutaneously transplantable squamous cell carcinoma. DNA from three transplantable cancers can induce NIH/ The 3T3 cells formed a foci whose frequency was slightly lower than that of human EJ bladder cancer cell lines, and the latter had Harvey and Kisotcn mouse sarcoma virus ras genes. Analysis of restriction endonucleases showed that DNA-induced conversion foci of mouse skin cancer cells existed. Another ras~H gene copy, this is not NIH/3T3