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AIM:To examine the effect of Eubacterium limosum(E.limosum)on colonic epithelial cell line in vitro,and toevaluate the effect of E.limosum on experimental colitis.METHODS:E.limosum was inoculated anaerobically andits metabolites were obtained.The growth stimulatoryeffect of the E.limosurn metabolites on T84 cells wasevaluated by SUDH activity,and the anti-inflammatoryeffect by IL-6 production.The change in mRNA of tolllike receptor 4(TLR4)was evaluated by real time PCR.Colitis was induced by feeding BALB/C mice with 2.0%dextran sodium sulfate.These mice received either 5%lyophilized E.limosum(n=7)or control diet(n=7).Seven days after colitis induction,clinical and histologicalscores,colon length,and cecal organic acid levels weredetermined.RESULTS:The E.limosum produced butyrate,acetate,propionate,and lactate at 0.25,1.0,0.025 and 0.07mmol/L,respectively in medium.At this concentration,each acid had no growth stimulating activity on T84cells; however,when these acids were mixed togetherat the above levels,it showed significantly highactivity than control.Except for lactate,these acidssignificantly attenuated IL-6 production at just 0.1mmol/L.In addition,under TNF-α stimulation,butyrateattenuated the production of TLR4 mRNA.The treatmentwith E.limosum significantly attenuated clinical and histological scores of colitis with an increase of cecalbutyrate levels,compared with the control group.CONCLUSION:E.limosum can ameliorate experimentalcolonic inflammation.In part,the metabolite ofE.limosum,butyrate,increases mucosal integrity andshows anti-inflammatory action modulation of mucosaldefense system via TLR4.
AIM: To examine the effect of Eubacterium limosum (E.limosum) on colonic epithelial cell line in vitro, and toevaluate the effect of E.limosum on experimental colitis. METHODS: E.limosum was inoculated anaerobically and are metabolites were. The growth stimulatory effect of the E.limosurn metabolites on T84 cells wasevaluated by SUDH activity, and the anti-inflammatory effect by IL-6 production. The change in mRNA of tolllike receptor 4 (TLR4) was evaluated by real time PCR. Colitis was induced by feeding BALB / C mice with 2.0% dextran sodium sulfate. The mice received either 5% lyophilized E.limosum (n = 7) or control diet (n = 7). Seven days after colitis induction, clinical and histologicalscores, colon length, and cecal organic acid Levels were determined .RESULTS: The E.limosum produced butyrate, acetate, propionate, and lactate at 0.25, 1.0, 0.025 and 0.07 mmol / L, respectively in medium. At this concentration, each acid had no growth stimulating activity on T84 cells; however, when these acids were mixed togethe rat the above levels, it showed significantly highactivity than control. Except for lactate, these acidssignificantly attenuated IL-6 production at just 0.1 mmol / L. In addition, under TNF-α stimulation, butyrateattenuated the production of TLR4 mRNA. limosum significantly attenuated clinical and histological scores of colitis with an increase of cecalbutyrate levels, compared with the control group .CONCLUSION: E.limosum can ameliorate experimentalcolonic inflammation.In part, the metabolite ofE.limosum, butyrate, increases mucosal integrity andshows anti-inflammatory action modulation of mucosaldefense system via TLR4.