植物细胞有丝分裂实验,按实验手册所述的染色方法,成功率很低。主要是采用整段根尖染色方法,染液很少渗入根尖细胞。所以显微观察效果较差。我们的作法是: 从固定液中取出洋葱根(1—2厘米),经解离和漂洗后,放在载玻片上,用镊子(或刀片)截取根尖乳白色部分(取乳白色部分的三分之二),剔除其余部分。用吸水纸吸去根尖周围的水。用镊子将乳白色部分进行捣烂,愈烂愈好。(这样才能使细胞充分分散,染色容易渗入各个细胞。)然后滴上一滴1%醋酸洋 Plant cell mitosis experiments, according to the staining method described in the laboratory manual, the success rate is very low. Mainly using the entire root tip staining method, the dye fluid rarely penetrates into the root tip cells. So microscopic observations are less effective. Our approach is: Remove the onion root (1-2 cm) from the fixative solution, dissociation and rinsing, and place it on a glass slide, and use tweezers (or blades) to cut the root tip of the white part (take a milky part of the three points (b), remove the rest. Remove the water around the root tip with absorbent paper. Use the tweezers to smash the milky white portion, and the better the rotting, the better. (In order to fully disperse the cells, the staining easily penetrates into each cell.) Then drop a drop of 1% acetic acid