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目的探讨HCV感染与血小板抗原基因之间的关系。方法提取114例抗-HCV ELISA双试剂检测均有反应性的献血者血清标本的RNA,做HCV RNA荧光定量检测,随访后将其分为HCV持续感染组(39例)和HCV自限清除组(75例);提取人基因组,用PCR-SSP方法对人血小板抗原(HPA)基因进行分型;比较HCV感染献血者与本地合格献血者和其他地区合格献血者HPA基因分型情况,分析HCV感染与HPA分型之间的关联性。结果与合格献血者相比,HCV感染献血者的HPA分型有不同表现(P>0.05);HPA基因多态性在HCV感染不同转归献血者中明显不同(P<0.05)。HCV持续感染组和对照组相比,HPA等位基因多态性有明显差异(P>0.05);HCV自限清除组和对照组的HPA-3a和HPA-3b基因频率分别为:40.70%vs 61.00%和59.30%vs 39.00%(P<0.01)。结论HPA-3的基因多态性与HCV易感性和自限清除是否具有一定的关联性,值得进一步研究。
Objective To investigate the relationship between HCV infection and platelet antigen genes. Methods A total of 114 anti-HCV ELISA reagents were used to detect RNA of serum samples from all donors who were reactive. HCV RNA was detected by quantitative real-time fluorescence quantitative PCR. The patients were divided into HCV persistent infection group (n = 39) and HCV self-limited elimination group (75 cases). Human genomic DNA was extracted and the human platelet antigen (HPA) gene was genotyped by PCR-SSP. The genotypes of HPA were compared between HCV infected blood donors, local donor blood donors and eligible blood donors in other regions. Correlation between infection and HPA typing. Results The HPA genotypes of HCV infected donors showed different manifestations (P> 0.05) compared with those of the eligible donors. The HPA gene polymorphisms were significantly different among the blood donors who had different outcomes of HCV infection (P <0.05). The frequencies of HPA-3a and HPA-3b genes in HCV self-limited group and control group were significantly higher than those in control group (40.70% vs 61.00% and 59.30% vs 39.00% (P <0.01). Conclusion The genetic polymorphism of HPA-3 is associated with HCV susceptibility and self-limiting clearance, which deserves further study.