论文部分内容阅读
为了研究丹参提取物对建鲤肝损伤生化指标的影响,选取体质健康、无伤的建鲤120条,随机分成6组,每组20条。5个处理组,1个对照组。每日饲喂2次,连续饲喂8周。I组(CCl4)、II组(0.5%丹参提取物)、III组(1%丹参提取物),按0.05 mL/10g体重体内注射30%CCl4;IV组(0.5%丹参提取物)、V组(1%丹参提取物)体内不注射CCl4,只注射同体积植物油;VI为空白对照组,也注射同体积植物油。注射72h后采集建鲤的血清及肝组织进行化验。结果表明:与I组(CCl4)相比,III组(1%丹参提取物)血清中丙氨酸转氨酶(GPT)、天冬氨酸转氨酶(GOT)、乳酸脱氢酶(LDH)的活性显著降低(P<0.05);总蛋白(TP)、白蛋白(ALB)的活性有所增加(P>0.05);超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)含量显著提高,丙二醛(MDA)含量显著降低(P<0.05);总抗氧化能力(T-AOC)和过氧化氢酶(CAT)含量显著提高(P<0.05)。而IV,V组的上述指标则无明显变化,这说明1%丹参提取物对于抗CCl4诱导的肝损伤有较好的效果。
In order to study the effect of Salvia miltiorrhiza extract on biochemical indexes of liver injury in Jian carp, 120 healthy carp Jianru were selected and randomly divided into 6 groups with 20 in each group. 5 treatment groups and 1 control group. Feeding twice daily and continuous feeding for 8 weeks. (CCl4), group II (0.5% Salvia miltiorrhiza extract), group III (Salvia miltiorrhiza extract 1%), and body weight 30% CCl4 in 0.05 mL / 10g body weight; Group IV (1% Salvia miltiorrhiza extract) in vivo injection of CCl4, only injected the same volume of vegetable oil; VI as a blank control group, but also injected with the same volume of vegetable oil. After 72 hours of injection, Jian carp serum and liver tissue were collected for testing. The results showed that the activity of alanine aminotransferase (GPT), aspartate aminotransferase (GOT) and lactate dehydrogenase (LDH) in group III (1% Salvia miltiorrhiza bunge extract) was significantly higher than that of group C (P <0.05); The activity of total protein (TP) and albumin (ALB) increased (P> 0.05); the activities of superoxide dismutase (SOD), glutathione peroxidase (P <0.05). The content of total antioxidant capacity (T-AOC) and catalase (CAT) increased significantly (P <0.05). However, there was no significant change in the above indexes in groups IV and V, indicating that the 1% Salvia miltiorrhiza extract has a good effect on anti-CCl4-induced liver injury.