Diagnosis of intestinal acariasis with avidin-biotin system enzyme-linked immunosorbent assay

来源 :World Journal of Gastroenterology | 被引量 : 0次 | 上传用户:DeadManWalk
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AIM:To explore the value of avidin-biotin system enzyme-linked immunosorbent assay (ABC-ELISA) in diagnosis ofintestinal acariasis.METHODS:Mite-specific IgG levels in serum of 48 patientswith intestinal acariasis were measured with ABC-ELISA.The sensitivity of this method was compared with that ofstaphylococcal protein A enzyme-linked immunosorbentassay (SPA-ELISA).RESULTS:The positive rate of mite-specific IgG detectedwith ABC-ELISA and SPA-ELISA was 89.58% (43/48) and56.25% (27/48),respectively.The positive rate with ABC-ELISA was statistically higher than that with SPA-ELISA(x~2=13.50,P<0.01).CONCLUSION:ABC-ELISA is an effective method for thediagnosis of intestinal acariasis. AIM: To explore the value of avidin-biotin system enzyme-linked immunosorbent assay (ABC-ELISA) in diagnosis of intestinal acariasis. METHODS: Mite-specific IgG levels in serum of 48 patients with intestinal acariasis were measured with ABC-ELISA. Sensitivity. this method was compared with that of the enzyme linked immunosorbent assay (SPA-ELISA) .RESULTS: The positive rate of mite-specific IgG detected with 89.58% (43/48) and 56.25% (43/48) ELISA was significantly higher than that with SPA-ELISA (x 2 = 13.50, P <0.01) .CONCLUSION: ABC-ELISA is an effective method for the diagnosis of intestinal mucositis
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