用水稻着丝粒重复序列RCS1为探针 ,与 30 72个克隆进行菌落杂交 ,得到了 32个阳性克隆 ,用RCS1与拟斯卑尔脱山羊草着丝粒重复序列Tcs2 5 0为探针进一步筛选 ,在 32个RCS1相关的阳性克隆中任选 10个克隆进行点杂交 ,分别有 6个和 5个阳性克隆 .为了克隆RCS1相关片段 ,依据RCS1的序列设计了三对引物 ,将引物 3从上述阳性克隆中扩增的一个 5 4 3bp的片段克隆测序 ,发现与水稻RCS1部分片段达到约 83%的同源 ,与大麦的反转座子 (Ty3 gypsy)部分序列同源性达到了 92 % ,与节节麦中着丝粒的整合酶基因部分序列同源性达到了 96 % ,命名为TBRCS1.TBRCS1可能是野生一粒小麦着丝粒区的组成部分 A total of 32 positive clones were obtained by colony hybridization with 30 72 clones using the rice centromere repeats RCS1 as a probe. Using RCS1 and Tcs250 as probes, We selected 10 clones out of 32 RCS1 positive clones for dot blot hybridization with 6 and 5 positive clones, respectively.For cloning RCS1-related fragments, three pairs of primers were designed according to the sequence of RCS1, primers 3 A 54 bp fragment amplified from the above positive clones was sequenced and found to be about 83% homologous to a partial RCS1 fragment of rice, with a homology of 92% to the partial sequence of Ty3 gypsy in barley , Which shared 96% homology with the integrase gene of Meloidogyne incognita and named TBRCS1.TBRCS1 may be an integral part of the wild wheat centromeric region