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目的探讨人肝细胞癌DNA干系倍体异质性的临床意义。方法选取45例肝细胞癌患者的归档蜡块,分别制备4μm、10μm的连续组织学切片,利用细胞图像分析仪测量细胞核DNA干系倍体值及其形态学参数。在4μm切片上测量细胞核DNA的平均光密度,在10μm切片上测量单个完整细胞核的体积。经细胞图像分析仪计算获得以单个完整细胞核体积为单位的DNA总量,以同一切片内正常淋巴细胞作为内参照,计算其DNA干系倍体值和异质性率。统计学分析DNA干系倍体异质性率与组织学分级、瘤体大小、淋巴结转移以及AFP表达水平的关系。结果肝细胞癌DNA干系倍体异质性率与组织学分级、瘤体大小、AFP表达水平有关联,与淋巴结转移无关联。结论组织原位法分析肝癌DNA干系倍体异质性对肝细胞癌的诊断、恶性度判定及预测预后均具有重要的参考价值。
Objective To investigate the clinical significance of DNA-ploidy haploid heterogeneity in human hepatocellular carcinoma. Methods Forty-five patients with hepatocellular carcinoma (HCC) were enrolled in this study. Serial 4μm and 10μm serial histological sections were prepared, and the cell nuclear DNA ploidy and morphological parameters were measured by cell image analyzer. The average optical density of nuclear DNA was measured on 4 μm sections and the volume of single intact nuclei was measured on 10 μm sections. The total amount of DNA in a single intact cell nucleus volume was calculated by the cell image analyzer and the normalized lymphocytes in the same slice were used as an internal reference to calculate the DNA dry ploidy value and the heterogeneity rate. Statistical analysis of the relationship between the rate of DNA ploidy heterogeneity and histological grade, tumor size, lymph node metastasis and the relationship between AFP expression levels. Results The frequency of hepatocellular carcinoma DNA ploidy heterogeneity was correlated with histological grade, tumor size and AFP expression, but not with lymph node metastasis. Conclusion Tissue in situ analysis of hepatocellular carcinoma DNA-dried ploidy heterogeneity has an important reference value for the diagnosis of hepatocellular carcinoma, the determination of malignancy and the prognosis of hepatocellular carcinoma.