Objective To understand the pre-pregnancy health knowledge and service needs among un-pregnant married women in Shanghai. Methods A total of 968 newly-married un-pregnant women in Changqiao, Lingyun sub-districts and Huajing town of Xuhui district, Shanghai, were recruited and inter- viewed by structured questionnaire to collect the information on pre-pregnancy health knowledge and service demands. Results 1) Newly-married women thought the contraceptive methods suitable for them was male condom(84.4%), followed by oral contraceptives(54%), emergent con- traceptive(52.5%) and contraceptive suppository (48.3%). 2) The score of pre-preg- nancy health knowledge was 54.7±14.5 among the subjects. The participants with junior college or a bachelor had more knowledge than those with junior or senior high school, and those who were engaged in medicine /education/science field had higher score. 3) They had higher knowing rate on the common knowledge, and the rate was above 80%. But the knowing rate on the knowledge of birth defect was low, and all below 50%. 4)A proportion of 52.7% of the respondents knew that there was a station for reproductive health service in their communities, but only 45.5% received the service from the station, 87.0% thought they had insufficient or scarce knowledge, 73.6% planned to consult before pregnancy and 63.6% were willing to attend the training courses on preparing pregnancy. Conclusion The participants had certain pre-pregnancy health knowledge, but still need being improved; in addition, they had higher needs on knowledge and service of birth defect prevention, at the same time they can’t make full use of the existing reproductive health institution. Objective To understand the pre-pregnancy health knowledge and service needs among un-pregnant married women in Shanghai. Methods A total of 968 newly-married un-pregnant women in Changqiao, Lingyun sub-districts and Huajing town of Xuhui district, Shanghai, were recruited and inter- viewed by structured questionnaire to collect the information on pre-pregnancy health knowledge and service demands. Results 1) Newly-married women thought the contraceptive methods suitable for them was male condom (84.4%), followed by oral contraceptives %), emergent con- tractptive (52.5%) and contraceptive suppository (48.3%). 2) The score of pre-preg- nancy health knowledge was 54.7 ± 14.5 among the subjects. The participants with junior college or a bachelor had more knowledge than those with junior or senior high school, and those who were engaged in medicine / education / science field had higher score. 3) They had higher knowing rate on the common knowledge, and the rate was above 80%. But the knowing rate on the knowledge of birth defect was low, and all below 50%. 4) A proportion of 52.7% of the respondents knew that there was a station for reproductive health service in their communities, but only 45.5% received the service from the station , 87.0% thought they had insufficient or scarce knowledge, 73.6% planned to consult before pregnancy and 63.6% were willing to attend the training courses on preparing pregnancy. Conclusion The participants had certain pre-pregnancy health knowledge, but still need being improved; in addition, they had higher needs on knowledge and service of birth defect prevention, at the same time they can not make full use of the existing reproductive health institution.