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目的:建立一种简便、可靠的完全脊髓横断伤模型的制作方法。方法:成年SD大鼠21只,随机分为横断伤组、挫伤组、对照组,每组7只。显露T12脊髓,横断组用尖刀将其完全横断。挫伤组150克.厘米力(gcf)冲击致伤。对照组仅显露脊髓,不做特殊处理。在不同时间对各组大鼠行行为学(BBB)评分及组织学评价。结果:横断组大鼠后肢功能评分在损伤后3～4周趋于稳定,损伤后4周时BBB评分为(5.14±0.80)分;挫伤组后肢功能评分进行性增高,4周时BBB评分达到(11.21±2.21)分;对照组2周左右恢复正常。横断组大鼠组织学观察见损伤区无脊髓组织残留,神经丝(NF)免疫组化染色未见神经纤维通过损伤区。结论:通过完全脊髓横断可以制备出稳定性好、一致性高的脊髓损伤模型。完全脊髓横断后,损伤恢复3～4周左右趋于稳定,可作为慢性脊髓损伤修复的移植期。
Objective: To establish a simple and reliable method of making complete transectional spinal cord injury model. Methods: Twenty-one adult SD rats were randomly divided into transverse injury group, contusion group and control group, with 7 rats in each group. The T12 spinal cord was revealed and the transection group was completely transected with a sharp knife. Contusion group 150 grams centimeter force (gcf) impact injury. The control group only exposed the spinal cord, do not do special treatment. The BBB scores and histological evaluation of rats in different groups at different time points. Results: The score of hindlimb function in the transected group tended to be stable at 3 to 4 weeks after injury, and the score of BBB was (5.14 ± 0.80) at 4 weeks after injury. The score of hindlimb function in the contusion group increased progressively. At 4 weeks, the BBB score reached (11.21 ± 2.21) points; control group returned to normal after about 2 weeks. In the transected group, there was no spinal cord tissue residue in the injured area. No nerve fibers passed through the lesion area in the neurofilament (NF) immunohistochemical staining. Conclusion: A complete spinal cord injury model with good stability and consistency can be prepared through complete spinal cord transection. After complete spinal cord transection, the injury tends to be stable after about 3 to 4 weeks of recovery and can be used as a graft for chronic spinal cord injury repair.