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于小鼠腹腔接种ＲＨ株弓形虫速殖子后２ｈ开始，采用乙胺嘧啶和复方磺胺甲口恶唑联合疗法持续治疗５２天，然后用ＰＣＲ与小鼠接种法检测小鼠脑和脾脏中的弓形虫。结果显示：ＰＣＲ与小鼠接种法的检出率分别为６３．６４％和１８．１８％（Ｐ＜０．０５）。提示ＰＣＲ在抗弓形虫药物筛选与疗效考核的体内试验中具有较大应用价值；使用上述两药联合治疗弓形虫病应有足够长的疗程。
The mice were inoculated intraperitoneally with RH strain Toxoplasma gondii 2h after the start of treatment with pyrimethamine and sulfamethoxazole combination therapy for 52 days, then PCR and mouse inoculation method to detect the mouse brain and spleen Toxoplasma. The results showed that the detection rates of PCR and inoculation were 63.64% and 18.18% respectively (P <0.05). Tip PCR in anti-Toxoplasma drug screening and efficacy evaluation of in vivo test has great value; the use of these two drugs should be treated with toxoplasmosis long enough course of treatment.