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目的:了解上海地区类流感病例中肠道病毒病原特征。方法:应用RD、Vero、Hep2细胞对2008年1月-12月收集的567份类流感咽拭子标本进行肠道病毒分离培养,并对分离的毒株进行肠道病毒Vp1区域扩增和测序,通过NC-BI BLAST服务器在线比对进行肠道病毒型别鉴定。结果:从567份咽拭子标本中分离到39株肠道病毒,全年阳性分离率占总分离标本数的6.88%。4月-7月期间获得分离毒株28株,阳性分离率占同期收集标本的21.05%。对所有分离到的肠道毒株进行测序比对,29株为COXB3型病毒、5株为COXA4型病毒、3株为COXA10型病毒,1株为COXA5型病毒和1株为COXA6型病毒。所有COXB3型毒株序列与GenBank网上当年公布的北京株、安徽株、广东株、广州株和日本株同源性达94%以上。结论:在上海地区2008年肠道病毒是引起上呼吸道感染的常见病原体之一,并以COXB3型病毒感染为主,还存在其他肠道病毒病原体感染。应用分子分型技术加强引起呼吸道感染的肠道病毒病原体监测可为相关疾病的流行或暴发提供预警信号,也为临床医生的正确诊断提供依据。
Objective: To understand the pathogenic characteristics of enterovirus in Shanghai flu-like cases. Methods: 567 influenza-like throat swabs collected from January 2008 to December 2008 were enrolled in this study. The isolated virus strains were amplified and sequenced from the Vp1 region of enterovirus , Enterovirus type identification by NC-BI BLAST server online comparison. Results: From the 567 throat swab specimens, 39 enteroviruses were isolated. The positive isolation rate in the whole year accounted for 6.88% of the total number of isolated specimens. From April to July, 28 isolates were obtained, accounting for 21.05% of the total samples collected during the same period. All the isolates were sequenced, 29 were COXB3, 5 were COXA4, 3 were COXA10, 1 was COXA5 and 1 was COXA6. All COXB3 strain sequences shared more than 94% homology with Beijing strain, Anhui strain, Guangdong strain, Guangzhou strain and Japanese strain published in GenBank online. Conclusion: In Shanghai, in 2008, enterovirus was one of the most common pathogens causing upper respiratory tract infection and was predominantly COXB3. Other enterovirus pathogens were also found. The application of molecular typing to enhance the detection of enterovirus pathogens causing respiratory infections may provide an early warning signal for the prevalence or outbreak of related diseases and provide the basis for the correct diagnosis of clinicians.