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目的 了解我国现有SPF鸡蛋对我国S191株麻疹病毒的可利用性。方法 比较S191株麻疹病毒 ,在我国 3个SPF种蛋供应厂商的 5个品系、6种SPF鸡蛋成纤维细胞上的细胞生长、病变特征和病毒效价方面的差异。结果 LOHMAN F、LOHMAN M、SPAFAS品系SPF鸡蛋的细胞生长发育形态和细胞病变较好 ,其中以SPAFAS为最佳 ;病毒效价 ,SPAFAS和LOHMAN M系在大于 5 .0 0LogCCID50 /ml的高滴度区间的样本数分别达 70 %和 71.96 % ,其中LOHMAN M系的样本都在 4.38LogCCID50 /ml以上的较高水平。结论 我国SPF鸡蛋的SPAFAS和LOHMAN M品系均适宜于S191株麻疹病毒的培养及麻疹疫苗生产
Objective To understand the availability of S191 measles virus in China with existing SPF eggs. Methods The S191 strain of measles virus was compared in terms of cell growth, pathological features and virus titer among five strains of three SPF egg suppliers in China and six SPF egg fibroblasts. Results The results showed that SPAFAS was the best, and the titer of virus titer, SPAFAS and LOHMAN M were higher than 5.0.0.0 LogCCID50 / ml in LOHMAN F, LOHMAN M and SPAFAS strains. The number of samples in the interval was 70% and 71.96%, respectively, of which, LOHMAN M samples were higher than 4.38LogCCID50 / ml. Conclusion The SPAFAS and LOHMAN M strains of SPF eggs are suitable for the culture of S191 measles virus and measles vaccine production