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目的:建立以高效液相色谱串联质谱电喷雾检测(LC-MS/MS)法测定人血浆中法罗培南浓度的方法。方法:采用API3200型四极杆串联质谱仪进行检测,色谱柱为Zorbax Eclipse C18,流动相为乙腈-水(70∶30),流速为0.4mL·min-1,离子源为电喷雾离子化源,检测方式为负离子方式,扫描方式为多重反应监测。结果:法罗培南血药浓度在50～2000ng·mL-1范围内线性关系良好(r=0.9991),最低检测浓度为1ng·mL-1(S/N>3);日内RSD<10%,日间RSD<12%,平均回收率为86.96%。结论:本方法简便、快速、精密度好,可用于法罗培南血药浓度的监测及药动学研究。
Objective: To establish a method for the determination of faropenem in human plasma by high performance liquid chromatography tandem mass spectrometry (LC-MS / MS). Methods: API3200 quadrupole tandem mass spectrometer was used for the detection. The chromatographic column was Zorbax Eclipse C18. The mobile phase consisted of acetonitrile-water (70:30) with a flow rate of 0.4 mL · min-1. The ion source was electrospray ionization , Negative ion detection mode, scanning for multiple reaction monitoring. Results: The plasma concentration of faropenem in the range of 50 ~ 2000ng · mL-1 was good (r = 0.9991), the lowest detection concentration was 1ng · mL-1 (S / N> 3) Between the RSD <12%, the average recovery was 86.96%. Conclusion: The method is simple, rapid and accurate. It can be used to monitor the plasma concentration of faropenem and study its pharmacokinetics.