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目的:探讨补脾益气方对哮喘模型大鼠肺组织核因子Kappa B(NF-κB)抑制剂的激酶β(the kinase of nuclear fac-tor-kappa B inhibitor β,IKKβ)的调控作用及对白介素8(IL-8)含量的影响。方法:SPF级雄性Wistar大鼠随机分为对照组(A)、哮喘组(B)、补脾益气方治疗组(C)。卵蛋白致敏和激发复制出哮喘模型,按照10 g.kg-1体重给予C组大鼠中药ig 1次/d,共21d。采用逆转录聚合酶链反应(RT-PCR)检测肺组织IKKβ的mRNA表达水平,采用免疫组化检测肺组织IKKβ的蛋白表达水平,采用酶联免疫吸附法(ELISA)测定支气管肺泡灌洗液IL-8的含量。结果:①肺组织IKKβ的mRNA的表达:B组显著高于A组(P<0.01);C组显著低于B组(P<0.05)。②肺组织IKKβ的蛋白表达水平:B组显著高于A组(P<0.01);C组显著低于B组(P<0.01)。③肺泡灌洗液IL-8含量:B组显著高于A组(P<0.01);C组显著低于B组(P<0.01)。结论:哮喘组大鼠肺组织IKKβmRNA和蛋白的表达水平显著增强,补脾益气方能有效抑制其表达,降低肺泡灌洗液IL-8含量,证明补脾益气方能有效抑制IKKβ信号转导的途径。
OBJECTIVE: To investigate the effect of Bupi Yiqi Recipe on the kinase of nuclear factor-kappa B (NF-κB) inhibitor of lung tissue and its effect on IKKβ Effect of interleukin 8 (IL-8) content. Methods: SPF male Wistar rats were randomly divided into control group (A), asthma group (B) and BuBu Yi Qi Fang treatment group (C). Ovalbumin sensitized and stimulated replication of asthma model, according to 10 g.kg-1 weight C group rats given ig 1 / d, a total of 21d. The mRNA expression of IKKβ in lung tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of IKKβ in lung tissue was detected by immunohistochemistry. The level of IKKβ mRNA in bronchoalveolar lavage fluid was determined by enzyme linked immunosorbent assay (ELISA) -8 content. Results: (1) The mRNA expression of IKKβ in lung tissue was significantly higher in group B than that in group A (P <0.01), while in group C was significantly lower than that in group B (P <0.05). ② The protein expression of IKKβ in lung tissue was significantly higher in group B than that in group A (P <0.01), but significantly lower in group C than that in group B (P <0.01). ③ The content of IL-8 in BALF was significantly higher in group B than that in group A (P <0.01), while in group C was significantly lower than that in group B (P <0.01). Conclusion: The expression of IKKβmRNA and protein in lung tissue of asthmatic rats was significantly increased. BuBu Yi Qi Fang can effectively inhibit the expression of ILK and decrease the content of IL-8 in bronchoalveolar lavage fluid. It is proved that BuKangYiQi can effectively inhibit IKKβ signaling Guide the way.