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本实验通过血管内皮细胞的体外培养,用RIA 法直接测定细胞培养液中,6-keto-PGF_(1a)和 TXB_2的含量并以此反映培养细胞合成分泌 PGI_2和 TXA_2的量。同时观察15Gyγ射线辐照前后的差别,以探索辐射损伤出血的原因。实验结果表明,在辐射后32小时中 TXA_2上升,PGI_2下降。正常组 PGI_2分泌量为 TXA_2的6倍。在血液内皮界面保持以 PGI_2为优势的平衡状态。γ-射线照射能激活内皮细胞中的环氧化酶和血栓素合成酶破坏这一平衡,使TXA_2的量增加,促使血小板在内皮细胞表面粘附聚集并释放平滑肌增殖因子,导致血管内皮细胞损伤,从而造成出血。
In this study, we measured the amount of 6-keto-PGF_ (1a) and TXB_2 in the cell culture medium by RIA in vitro and reflected the amount of PGI_2 and TXA_2 synthesized by cultured cells. At the same time, we observed the difference of 15Gy γ ray before and after irradiation to explore the cause of radiation injury hemorrhage. The experimental results showed that TXA_2 increased and PGI_2 decreased in 32 hours after irradiation. Normal group PGI_2 secretion of TXA_2 6 times. Maintaining PGI2 as the predominant balance at the blood endothelial interface. γ-ray irradiation can activate the balance of cyclooxygenase and thromboxane synthase in endothelial cells, increase the amount of TXA_2, promote the adhesion and aggregation of platelets on the surface of endothelial cells and release the smooth muscle proliferation factor, resulting in the injury of vascular endothelial cells , Resulting in bleeding.