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目的:建立测定黄栀巴布贴中胆酸含量的检测方法。方法:采用高效液相法蒸发光散射检测器(HPLC-ELSD)测定,色谱条件为:Kromasil C_(18)柱(250 mm×4.6 mm,5μm);流动相为甲醇-乙腈-2%醋酸溶液(60:10:30);蒸发光散射检测器检测参数:漂移管温度75℃,载气(N_2)流速2.0 ml·min~(-1)。结果:胆酸在0.098～1.960μg(r=0.999 1)范围内线性关系良好,平均加样回收率为97.7%,RSD为1.5%。结论:该法操作简便,结果准确,重复性好,可以控制黄栀巴布贴的含量。
Objective: To establish a method for the determination of bile acid in yellow dampness patch. Methods: The HPLC conditions were as follows: Kromasil C 18 column (250 mm × 4.6 mm, 5 μm); the mobile phase consisted of methanol-acetonitrile-2% acetic acid solution (60:10:30); detection parameters of evaporative light scattering detector: drift tube temperature 75 ℃, flow rate of carrier gas 2.0 ml · min ~ (-1). Results: The linearity of cholic acid in the range of 0.098-1.960 μg (r = 0.999 1) was good with an average recovery of 97.7% and a RSD of 1.5%. Conclusion: The method is simple, accurate, reproducible, and can control the content of Huangzhu Babu.