取新生大鼠小脑皮质进行体外神经细胞培养 ,用兴奋性氨基酸建立神经元变性损害模型 ,并在培养液中加入不同浓度胶质细胞衍生性神经营养因子 (GDNF) ,观察其对兴奋性氨基酸毒性的影响及受损神经元的恢复。结果表明 :在培养液中加入 GDNF可明显减轻小脑皮质神经元变性、死亡 ,并呈正量效关系。提示 GDNF可拮抗兴奋性氨基酸神经毒性 ,对小脑皮质神经元有保护、促进其存活和功能恢复作用 Cultured neuronal cultures of neonatal rat cerebellar cortex were established. Neuronal degeneration damage models were established by excitatory amino acids. Different concentrations of glial cell-derived neurotrophic factor (GDNF) were added to the culture medium to observe their effects on excitatory amino acid toxicity Effects of impaired neurons on recovery. The results showed that adding GDNF into culture medium could significantly reduce the degeneration and death of neurons in the cerebellar cortex, and showed a positive dose-effect relationship. It is suggested that GDNF can antagonize the excitatory amino acid neurotoxicity and protect the cerebellar cortical neurons and promote their survival and function recovery