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目的:研究灯盏花素对AD模型小鼠学习记忆的影响。方法:选用东莨菪碱造模的雄性KM小鼠,用小鼠跳台仪测试行为学指标,采用黄嘌呤氧化酶比色法测定超氧化物歧化酶(SOD)活性、硫代巴比妥酸法测定脂质过氧化产物丙二醛(MDA)含量、三硝基苯比色法测定脑内胆碱酯酶(AchE)活性。结果:灯盏花素能明显降低模型小鼠错误次数,延长错误潜伏期;显著提高血清和脑组织SOD活性,降低MDA含量;明显降低脑组织AchE活性。结论:灯盏花素能改善造模小鼠的学习记忆功能,其机制可能与提高SOD活性、降低脑组织脂质过氧化物的含量、抑制AchE活性有关。
Objective: To study the effects of breviscapine on learning and memory in AD model mice. METHODS: Male KM mice modeled with scopolamine were selected, the behavioral parameters were measured with a mouse odometer, the activity of superoxide dismutase (SOD) was measured with the xanthine oxidase colorimetric method, and the thiobarbituric acid method was used to measure the lipids. The content of malondialdehyde (MDA) in the product of peroxidation and the activity of cholinesterase (AchE) in brain were measured by trinitrobenzene colorimetry. RESULTS: Breviscapine could significantly reduce the number of errors in model mice and prolong the error latency; significantly increase the activity of SOD and decrease the content of MDA in serum and brain; and significantly decreased AchE activity in brain tissue. CONCLUSION: Breviscapine can improve the learning and memory function of model mice. The mechanism may be related to the increase of SOD activity, the decrease of lipid peroxide content in brain tissue, and the inhibition of AchE activity.