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目的探讨增强Zeste同源2(EZH2)与年龄相关性胰岛细胞功能的关系。方法健康SD大鼠分为1、6、12及24月龄组,每组各6只。取大鼠胰腺,行免疫组化染色。Western blot检测胰岛内EZH2及胰岛素水平。行Ki67染色及TUNEL染色分别检测胰岛细胞增殖及凋亡。结果随年龄增加,胰岛内EZH2表达逐渐减少:1月龄组(0.22516±0.03091);6月龄组(0.18427±0.02315);12月龄组(0.03165±0.01675);24月龄组0(P<0.01)。胰岛细胞增殖逐渐减少:6月龄组(0.36±0.03)%;12月龄组(0.61±0.02)%;24月龄组(0.12±0.02)%(P<0.01)。胰岛细胞凋亡逐渐增加:12月龄组(0.02±0.03)%;24月龄组(0.09±0.04)%(P<0.01)。胰岛内胰岛素水平:1月龄组(206.5±27.8)mmol/L;6月龄组(267.4±25.3)mmol/L;12月龄组(376.2±31.9)mmol/L;24月龄组(187.8±25.1)mmol/L(P<0.01)。结论随年龄增加,胰岛细胞逐渐衰老,胰岛细胞内EZH2表达逐渐减少,伴随着胰岛细胞增殖能力下降,凋亡增加,胰岛素水平逐渐减低。
Objective To explore the relationship between enhanced Zeste homology 2 (EZH2) and age-related islet cell function. Methods Healthy SD rats were divided into 1, 6, 12 and 24 month old groups, 6 in each. The rat pancreas was taken for immunohistochemical staining. Western blot detection of islet EZH2 and insulin levels. Ki67 staining and TUNEL staining were used to detect islet cell proliferation and apoptosis respectively. RESULTS: The expression of EZH2 in islets decreased gradually with the increase of age: 1 month old group (0. 22516 ± 0. 03091), 6 month old group (0.18427 ± 0. 02315), 12 month old group (0. 03165 ± 0. 01675), 24 month old group 0 0.01). The islet cell proliferation gradually decreased: 6 months old group (0.36 ± 0.03%); 12 months old group (0.61 ± 0.02%); 24 months old group (0.12 ± 0.02)% (P <0.01). Apoptosis of pancreatic islets increased gradually: 12 months old (0. 02 ± 0.03%); 24 months old (0.09 ± 0.04)% (P <0.01). Insulin levels in the insulina were (206.5 ± 27.8) mmol / L in 1-month group, 267.4 ± 25.3 mmol / L in 6-month group and 376.2 ± 31.9 mmol / L for 12- ± 25.1) mmol / L (P <0.01). Conclusion With the increase of age, the islet cells gradually senesce, the expression of EZH2 in islet cells gradually decreases, accompanied with the decrease of the islet cell proliferation ability, the increase of apoptosis and the decrease of the insulin level.