目的:研究疏肝益阳(SGYY)胶囊治疗勃起功能障碍的分子机制。方法:选取3月龄成年雄性SD大鼠60只,采用双侧髂内动脉结扎法制造血管性勃起障碍模型。设假手术对照组、模型组、西药西地那非组(10.5mg.kg-1.d-1)、SGYY大剂量治疗组(1g.kg-1.d-1)、SGYY小剂量治疗组(0.5g.kg-1.d-1),每组12只,灌胃给药,疗程30d。取大鼠血浆,酶联免疫吸附(ELISA)法检测内皮素-1(ET-1)含量;取阴茎组织,实时荧光定量PCR(real-time PCR)法检测ET和连接蛋白43(CX43)mRNA表达。结果:3个治疗组与模型组比较,血浆ET-1含量显著降低(P<0.05,P<0.01),阴茎组织ET mRNA表达显著降低(P<0.01),CX43 mRNA表达显著升高(P<0.01)。结论:疏肝益阳胶囊可显著降低双侧髂内动脉结扎法制造的动脉性勃起障碍大鼠血浆ET-1含量和阴茎组织ET基因表达,并能显著增加阴茎组织CX43基因表达,这可能是其治疗血管性ED的机制之一。 Objective: To study the molecular mechanism of Shugan Yiyang (SGYY) capsule in the treatment of erectile dysfunction. Methods: Sixty adult male Sprague Dawley rats aged 3 months were enrolled in this study. The model of vasular erectile dysfunction was established by bilateral iliac artery ligation. The sham operation control group, model group, western medicine sildenafil group (10.5mg.kg-1.d-1), SGYY high-dose treatment group (1g.kg-1.d-1), SGYY low-dose treatment group (0.5g.kg-1.d-1), each group of 12, intragastric administration, treatment 30d. The content of ET-1 was detected by enzyme-linked immunosorbent assay (ELISA) in the plasma of rats. The penile tissue was collected and the expression of ET and connexin 43 (CX43) mRNA was detected by real-time PCR expression. Results: Compared with the model group, ET-1 levels in the three treatment groups were significantly decreased (P <0.05, P <0.01), ET mRNA expression in the penis tissue was significantly decreased, and CX43 mRNA expression was significantly increased (P < 0.01). Conclusion: Shugan Yiyang Capsule can significantly reduce the content of ET-1 in plasma and the expression of ET gene in penile tissue in rats with arterial erectile dysfunction induced by bilateral internal iliac artery ligation, and significantly increase the expression of CX43 in penile tissue, which may be the result of One of the mechanisms for the treatment of vascular ED.