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目的 :用分子生物学的方法探讨HLA DRB1 等位基因与免疫遗传的关系。方法 :应用序列特异性引物聚合酶链反应 (PCR SSP)基因分型方法鉴定HLA DRB1 与天津地区支气管哮喘 (asthmaAS)的相关性。结果 :表明支气管哮喘患者的DR3 较健康对照明显升高 (11 76 %vs3 33% ,χ2 =4 2 5 ,P<0 0 5 ) ;而DR6(1 3 ) 在支气管哮喘患者中虽有升高 ,但无统计学意义 (8 82 %vs 4 44 % ,χ2 =1 2 5 ,P >0 0 5 ) ;支气管患者DR2 (1 5) 频率明显降低 (5 88%vs 16 6 7% ,χ2 =4 2 6 ,P <0 0 5 )。结论 :表明DR3 与支气管哮喘易感性呈正相关 ,而DR2 (1 5) 与支气管哮喘抗性相关
Objective: To explore the relationship between HLA DRB1 alleles and immune inheritance by molecular biology method. Methods: The relationship between HLA DRB1 and asthma AS in Tianjin was identified by PCR SSP genotyping. Results: The DR3 in bronchial asthma patients was significantly higher than that in healthy controls (11 76% vs 33%, χ2 = 4255, P <0.05), while DR6 (13) was elevated in patients with bronchial asthma (8 82% vs 444%, χ2 = 1255, P> 0.05). The frequency of DR2 (15) was significantly decreased in bronchial patients (58.8% vs 16.67%, χ2 = 4 2 6, P <0 0 5). Conclusions: This suggests that DR3 is positively associated with susceptibility to bronchial asthma, whereas DR2 (15) is associated with bronchial asthma resistance