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为了确定商陆口服液的最佳提取工艺,本试验首先对商陆中商陆皂苷甲(Es A)的含量测定方法进行了研究,然后采用水煎法、水提醇沉法(乙醇终浓度分别为60%、70%、80%和90%)、减压干燥法、渗漉法(乙醇浓度分别为30%、50%、70%、80%和90%)4种提取方法,比较了各提取物中Es A的含量。结果表明:采用C18柱,蒸发光散射检测器进行检测;流动相为甲醇-0.4%冰醋酸溶液(70∶30);流速为1.0 m L/min;柱温为30℃;衰减值(Attenuation)为7;雾化温度(Neb)为45℃;蒸发温度(Eva)为60℃;进样量为10μL;Es A的线性范围在1.03~5.02μg,平均回收率为99.20%,用90%的乙醇渗漉提取效果好。说明Es A含量测定方法稳定可靠,可作为质量控制的指标,提取方法简单易行,提取率高。
In order to determine the optimum extraction process of the pokeweed liquid, this study firstly studied the method of determination of Es A in P. fortunei, and then used water decoction method, water extraction and alcohol precipitation method (final concentration of ethanol Respectively, 60%, 70%, 80% and 90%), vacuum drying and percolation method (ethanol concentration of 30%, 50%, 70%, 80% and 90% Es A content in each extract. The results showed that C18 column and evaporative light scattering detector were used for the detection. The mobile phase consisted of methanol-0.4% acetic acid solution (70:30), the flow rate was 1.0 m L / min, the column temperature was 30 ℃, the attenuation value (Attenuation) Was 7; Neb was 45 ℃; Eva was 60 ℃; injection volume was 10μL; the linear range of Es A was 1.03 ~ 5.02μg, the average recovery was 99.20% Ethanol percolation extraction effect. It shows that the method of determination of Es A is stable and reliable and can be used as an indicator of quality control. The extraction method is simple and easy, and the extraction rate is high.