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国的乙型肝炎病毒（ＨＢＶ）Ｃ基因调节序列存在较多变异，为探讨在诸多变异中，Ｔ１７６２Ａ１７６４变异对Ｃ区启动子（ＣＰ）活性究竟起何作用。方法用克隆及ＰＣＲ产物直接测序，检测Ｔ１７６２Ａ１７６４变异;并将含调节序列的ＰＣＲ产物插入到氯霉素乙酰转移酶（ＣＡＴ）表达质粒中，转染ＨｅｐＧ2细胞并瞬时表达。结果通过比较野生株及变异株在ＣＰ区序列及ＣＡＴ表达水平显示，Ｔ１７６２Ａ１７６４变异在使ＣＡＴ表达下调中起主要作用。结论Ｔ１７６２Ａ１７６４变异在使ＣＰ活性下调中起主要作用。
There are many variations in the regulatory sequences of hepatitis B virus (HBV) C gene in China. To explore the role of T1762A1764 mutation in C-region promoter (CP) activity in many variations. Methods The clones and PCR products were directly sequenced to detect the variation of T1762A1764. The PCR products containing the regulatory sequences were inserted into the expression plasmid of chloramphenicol acetyltransferase (CAT) and transfected into HepG2 cells for transient expression. Results The comparison of the sequences of CAT and the expression of CAT in wild and mutant strains showed that the variation of T1762A1764 played a major role in down-regulation of CAT expression. Conclusion T1762A1764 mutation plays a major role in down-regulation of CP activity.