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以番禺香蕉、越南香蕉(Musa acuminata AAA)及大蕉(M.paradisiaca ABB)的座果后顶端花序轴1cm 处的幼嫩部分为外植体,用0.1%升汞消毒10分钟,以无菌水冲洗3次后,将外植体切成小片,先接种在 MS 培养基附加有2mg/l 的2,4-D,2,4,5-T,IAA,NAA,GA_3,ABA,KT 及 BTP 及其相互配合的不同的培养基中预培养一段时间。为了解花序不同部位(不同发育年龄)及不同组织对离体培养的形态发生的影响,部分材料接种时特别标记了花序片段的上下位置,或经预培养一段时间后,将花序的
Using the tender parts of the top inflorescence axis of Panyu banana, Musa acuminata AAA and M.paradisiaca ABB as explants, the explants were disinfected with 0.1% mercuric chloride for 10 minutes, After washing with water for three times, the explants were cut into small pieces and inoculated into MS medium supplemented with 2mg / l of 2,4-D, 2,4,5-T, IAA, NAA, GA_3, ABA, KT and BTP, and their different matrices for a period of time. In order to understand the different parts of inflorescence (different developmental ages) and the influence of different tissues on the morphology of in vitro culture, some materials were inoculated with the upper and lower positions of the inflorescences, or preincubated for a period of time,