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目的高脂膳食结合小剂量链脲佐菌素建立中国地鼠的高糖高脂模型。方法采用高脂膳食喂养中国地鼠38周,结合链脲佐菌素(STZ)20 mg·kg-1腹腔注射。将中国地鼠分3组:普通饮食组(KB)、普通饮食STZ组(KSTZ)、高脂膳食STZ组(HSTZ),定期观察体重、血糖的变化,38周后取血清测定TC、LDL、HbA1c、NO活力和GSH水平。结果在4周、19周时HSTZ组血糖较KB组明显升高;38周时KSTZ组血糖、HbA1c较KB组显著升高,KSTZ组TC较KB组明显升高,HSTZ组TC、LDL均显著高于KB组和KSTZ组。NO活力KSTZ组明显高于KB组和HSTZ组,GSH水平HSTZ组显著高于KB组和KSTZ组,但后2组间无差异。结论多次低量STZ有缓慢升高中国地鼠血糖,高脂饲养升高血脂但未加重高血糖,该模型具有一定的氧化应激损伤。
Objective To establish a high-sugar and high-fat model of Chinese hamster by combining high-fat diet with low-dose streptozotocin (STZ). Methods Chinese hamsters were fed with a high-fat diet for 38 weeks and injected intraperitoneally with streptozotocin (STZ) 20 mg · kg-1. The Chinese hamster was divided into three groups: normal diet group (KB), normal diet STZ group (HSTZ) and high fat diet STZ group (HSTZ group). The changes of body weight and blood glucose were observed regularly. Serum TC, LDL, HbA1c, NO activity and GSH levels. Results At 4 weeks and 19 weeks, blood glucose in HSTZ group was significantly higher than that in KB group. At 38 weeks, blood glucose and HbA1c in KSTZ group were significantly higher than those in KB group. TC in KSTZ group was significantly higher than that in KB group. TC and LDL in HSTZ group were significantly Higher than KB group and KSTZ group. NO activity in KSTZ group was significantly higher than that in KB group and HSTZ group. GSH level in HSTZ group was significantly higher than that in KB group and KSTZ group, but there was no difference between the two groups. CONCLUSIONS: After repeated low doses of STZ, blood glucose was slowly increased in Chinese hamsters. High-fat diets increased blood lipids but did not aggravate hyperglycemia. This model has some oxidative stress injury.