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采用0.5％～1.0％低血清培养法较好地模拟了小剂量rhTNF-α(50U/ml)对人早幼粒白血病细胞株HL-60的增殖抑制效应,但未发现促分化作用,对细胞株c-myc基因的表达亦无明显影响;而TNF(50μ/ml)具有诱导HL-60细胞分化和抑制细胞增殖的作用,且显著抑制其c-myc癌基因的表达水平。结果表明c-myc基因表达减低主要与TNF诱导HL-60白血病细胞分化相关,并非细胞增殖受抑的结果,对癌基因表达水平的调控可能是小剂量TNF发挥分化诱导作用的重要机制。
The low-dose serum culture method with 0.5%～1.0% better simulated the effect of low dose of rhTNF-α (50U/ml) on the proliferation of human promyelocytic leukemia cell line HL-60, but no cell differentiation was observed. The expression of c-myc gene also had no significant effect, while TNF (50μ/ml) induced the differentiation of HL-60 cells and inhibited cell proliferation, and significantly inhibited the expression of c-myc oncogene. The results showed that the decrease of c-myc gene expression was mainly related to TNF-induced differentiation of HL-60 leukemia cells, not the result of inhibition of cell proliferation. Regulation of oncogene expression levels may be an important mechanism for low-dose TNF to exert differentiation induction.