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目的了解北京地区男男性接触者(MSM)人群HIV-1流行亚型分布及其变化趋势。方法 2005～2009年在北京市开展4次MSM人群HIV-1抗体筛查及确证试验,阳性者采外周血,提取RNA,通过逆转录及巢式PCR扩增HIV-1的env基因,并进行系统发育树分析以确定其亚型。结果经系统发育树分析,北京地区MSM人群中HIV-1的主要流行亚型是欧美B、CRF01_AE和CRF07_BC亚型,2009年还检出C亚型。2005～2009年该人群中HIV-1主要流行亚型的趋势发生变化:欧美B亚型所占比例下降,CRF01_AE和CRF07_BC亚型所占比例上升。结论在北京地区MSM人群中,HIV-1的流行亚型逐渐呈多元性,而且主要亚型的流行趋势已发生改变。
Objective To understand the distribution and trend of HIV-1 subtypes in MSM in Beijing. Methods Four MSM HIV-1 antibody screening and confirmation tests were conducted in Beijing from 2005 to 2009. Positive samples were collected from peripheral blood, RNA was extracted, and the env gene of HIV-1 was amplified by reverse transcription and nested PCR. Phylogenetic tree analysis to determine its subtype. Results According to the phylogenetic tree analysis, the major epidemic subtypes of HIV-1 in MSM population in Beijing were the European and American B, CRF01_AE and CRF07_BC subtypes. In 2009, the C subtype was also detected. The trend of HIV-1 major epidemic subtypes in this population from 2005 to 2009 changed: the proportion of European and American subtype B decreased, and the proportion of CRF01_AE and CRF07_BC subtypes increased. Conclusion Among the MSM population in Beijing, the prevalence of HIV-1 is gradually diversified, and the prevalence of major subtypes has changed.