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采用气相色谱法,对蜂蜡主要成分二十八烷醇、三十烷醇进行了含量测定。测定条件为1m×3mm不锈钢柱,以ChromosorbWAWDMCS为填料,1.5%OV-17和1.5%SE-30为固定相;柱温为295℃;进样品、检测器(FID)温度分别为320℃。结果表明,方法准确、稳定、重现性好,可作为蜂蜡总烷醇或其制剂的含量测定。
By gas chromatography, the main components of beeswax octacosanol, triacontanol were determined. The conditions were 1m × 3mm stainless steel column, ChromosorbWAWDMCS as filler, 1.5% OV-17 and 1.5% SE-30 as stationary phase. The column temperature was 295 ℃. The temperature of sample and detector (FID) 320 ° C. The results show that the method is accurate, stable and reproducible. It can be used as the determination of total alkanol or its preparation.