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通过氮磷添加控制试验,对湖南城步(CB)、湖北秭归(ZG)、河南内乡(NX)、河北临城(LC)和北京平谷(PG)5个种源的栓皮栎1年生幼苗的叶片温度耐性指标(耐寒性、耐热性及耐温范围)及相关生理指标(叶片养分浓度、非结构性碳水化合物及其组分和养分利用效率)进行测定,研究养分对植物种内不同地理种源的温度耐性影响.结果表明:不同种源的栓皮栎养分吸收与氮磷利用效率对氮磷添加存在显著差异但地理趋势不明显.PG在N、P及其二者复合添加的情况下均具有较高的氮磷利用效率,NX则在不同处理下磷利用效率均处于最低水平,CB在磷添加的情况下对磷的利用效率最高.与对照相比,养分(N、P或NP)添加能明显增加PG和LC的耐寒性,但N添加降低了NX的耐寒性.同时,养分添加在一定程度上增加了ZG、NX和LC的耐热性.耐温范围与耐寒性相反,表现为养分添加降低了PG和LC的温度耐受范围,而增加了NX的温度耐受范围.偏相关分析表明,耐寒性与叶片可溶性糖浓度呈显著正相关;耐热性与叶片P呈显著负相关,与N/P呈显著正相关;叶片耐温范围与叶片物质浓度无显著相关性.因此,不同地理种源对养分利用程度及其效率可能与源生地土壤环境条件有关,表现出一定的局域适应特征,从而增加了温度耐性对养分响应的复杂程度.
Through the control experiments of nitrogen and phosphorus, the annual seedlings of one-year-old seedlings of Quercus variabilis (Quercus variabilis) were collected from five provenances in Chengbu (CB), Zigui (ZG), Neixiang (Henan), Lincheng (LC) and Pinggu (Leaf temperature, non-structural carbohydrates, their components and nutrient use efficiency) of leaves under different temperature were measured to study the effects of nutrients on plant temperature Geographical provenance.The results showed that nutrient absorption and N, P use efficiency of Quercus variabilis had significant differences on the addition of N and P, but the geographical trend was not obvious.When N, P and their combination were added Under high phosphorus use efficiency, NX had the lowest phosphorus use efficiency under different treatments and CB had the highest phosphorus use efficiency under phosphorus supplementation.Compared with the control, nutrient (N, P or NP) could significantly increase the cold tolerance of PG and LC, but the addition of N reduced the cold tolerance of NX, while the addition of nutrients increased the heat resistance of ZG, NX and LC to a certain extent. , Showed that nutrient addition reduced the temperature tolerance of PG and LC However, the temperature tolerance range of NX was increased. Partial correlation analysis showed that there was a significant positive correlation between cold tolerance and leaf soluble sugar concentration. Heat tolerance was negatively correlated with leaf P and positively correlated with N / P. There was no significant correlation between temperature range and leaf matter concentration.Therefore, the degree of nutrient utilization and its efficiency of different geographical provenances may be related to the soil environmental conditions of the habitat, showing some local adaptation characteristics, thus increasing the temperature tolerance of nutrients The complexity of the response.