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目的研究载有多种癌变相关基因的１７号染色体在不同器官、不同组织类型肿瘤单个细胞内拷贝数的变化规律。方法以正常外周血淋巴细胞为对照，采用ＦＩＳＨ方法检测各细胞株１７号染色体拷贝数。结果１７号染色体在各细胞株单个细胞中拷贝数表现为：ＳＫＯＶ３以六倍体为主，占４８％，七倍体率占２３％；３ＡＯ以三倍体为主，占９３％；ＯＶ２以四倍体为主，占６７％；ＭＧＣ８０３以六倍体为主，占６４％；ＢＧＣ８２３以二倍体为主，占８３％；ＳＧＣ７９０１以三倍体为主，占８０％；ＰＡＭＣ８２以五倍体为主，占７８％。结论各细胞系均无１７号染色体缺失，反呈扩增趋势。
Objective To study the variation of the copy number of chromosome 17 in different organs and different tissue types of chromosome 17 carrying a variety of cancer-associated genes. Methods Normal peripheral blood lymphocytes were used as controls. FISH was used to detect chromosome 17 copies of each cell line. RESULTS: The copy number of chromosome 17 in individual cell lines was: SKOV3 was predominantly hexaploid, accounting for 48%, and heptaploid rate was 23%; 3AO was mainly triploid, accounting for 93%; OV2 was Tetraploid predominates, accounting for 67%; MGC803 is predominantly hexaploid, accounting for 64%; BGC823 is predominantly diploid, accounting for 83%; SGC7901 is predominantly triploid, accounting for 80%; PAMC82 is fivefold The main body, accounting for 78%. Conclusion There is no deletion of chromosome 17 in all cell lines.